View source for Unknown Organization (20250092448). METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATIONS

=METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATIONS=


==Organization Name==

[[:Category:Unknown Organization|Unknown Organization]]


[[Category:Unknown Organization]]

==Inventor(s)==

[[:Category:Shoulian Dong of Mountain View CA US|Shoulian Dong of Mountain View CA US]][[Category:Shoulian Dong of Mountain View CA US]]

[[:Category:Christopher X Dong of Mountain View CA US|Christopher X Dong of Mountain View CA US]][[Category:Christopher X Dong of Mountain View CA US]]

[[:Category:Wiliam X Dong of Mountain View CA US|Wiliam X Dong of Mountain View CA US]][[Category:Wiliam X Dong of Mountain View CA US]]

==METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATIONS==


This abstract first appeared for US patent application 20250092448 titled 'METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATIONS

==Original Abstract Submitted==

described herein are methods and compositions that provide highly efficient nucleic acid amplification. in some embodiments, dna oligonucleotide containing ribonucleotide is used as primer in the presence of rnase h2. in pcr reactions, this allows a greater than 2-fold increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional pcr. pcr product thus generated is used for convenient cloning. in isothermal reactions, this reduces the time needed for target detection. in some embodiment, probe containing ribonucleotide is used to monitor amplification reaction without relying on 5′-nuclease activity of dna polymerase. the method and composition enable easy and fast detection of nucleic acid targets, enabling quick detection of genetic variations and pathogens such as sars-cov-2.



[[Category:C12Q1/6844]]
[[Category:C12Q1/6876]]
[[Category:CPC_C12Q1/6844]]