Unknown Organization (20250092448). METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATIONS
METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATIONS
Organization Name
Inventor(s)
Shoulian Dong of Mountain View CA US
Christopher X Dong of Mountain View CA US
Wiliam X Dong of Mountain View CA US
METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATIONS
This abstract first appeared for US patent application 20250092448 titled 'METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATIONS
Original Abstract Submitted
described herein are methods and compositions that provide highly efficient nucleic acid amplification. in some embodiments, dna oligonucleotide containing ribonucleotide is used as primer in the presence of rnase h2. in pcr reactions, this allows a greater than 2-fold increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional pcr. pcr product thus generated is used for convenient cloning. in isothermal reactions, this reduces the time needed for target detection. in some embodiment, probe containing ribonucleotide is used to monitor amplification reaction without relying on 5′-nuclease activity of dna polymerase. the method and composition enable easy and fast detection of nucleic acid targets, enabling quick detection of genetic variations and pathogens such as sars-cov-2.
