QIAGEN GmbH (20250011853). METHOD OF EXAMINING A NUCLEIC ACID AMPLIFICATION PRODUCT

METHOD OF EXAMINING A NUCLEIC ACID AMPLIFICATION PRODUCT

Organization Name

QIAGEN GmbH

Inventor(s)

Ronny Kellner of Bad Honnef DE

METHOD OF EXAMINING A NUCLEIC ACID AMPLIFICATION PRODUCT

This abstract first appeared for US patent application 20250011853 titled 'METHOD OF EXAMINING A NUCLEIC ACID AMPLIFICATION PRODUCT

Original Abstract Submitted

the invention relates to a method for examining a nucleic acid amplification product, comprising the steps of a. providing an amplification product stemming from a preferably exponential amplification comprising i. an upstream and downstream primer region as well as optionally and preferably, ii. an amplified target region between said upstream and downstream primer region, b. examining the amplification product by amplifying said amplification product in a pcr reaction, wherein i. the upstream and downstream pcr primers in said pcr reaction bind in the upstream and downstream primer region of the amplification product, ii. a first and a second oligonucleotide probe are placed downstream of the first and second pcr primers and within the upstream and the downstream primer region, c. performing a pcr reaction and examining the newly formed amplification product, wherein the upstream and downstream primer regions of step a.i.) have a length of under 35 nucleotides and over 20 nucleotides.