Microsoft technology licensing, llc (20250146053). OLIGONUCLEOTIDE ASSEMBLY USING pH BASED ELECTRODE CONTROLLED HYBRIDIZATION
OLIGONUCLEOTIDE ASSEMBLY USING pH BASED ELECTRODE CONTROLLED HYBRIDIZATION
Organization Name
microsoft technology licensing, llc
Inventor(s)
Yuan-Jyue Chen of Seattle WA US
Bichlien Hoang Nguyen of Seattle WA US
Karin Strauss of Seattle WA US
Jake Allen Smith of Seattle WA US
OLIGONUCLEOTIDE ASSEMBLY USING pH BASED ELECTRODE CONTROLLED HYBRIDIZATION
This abstract first appeared for US patent application 20250146053 titled 'OLIGONUCLEOTIDE ASSEMBLY USING pH BASED ELECTRODE CONTROLLED HYBRIDIZATION
Original Abstract Submitted
electrode controlled hybridization is used to change local ph and selectively assemble oligonucleotide complexes on the surface of a microelectrode array. the oligonucleotide complexes have sticky ends that provide locations for subsequent oligonucleotide complexes to hybridize. the order in which specific oligonucleotide complexes are joined together encodes information. controlled activation of individual electrodes in the microelectrode array creates negative voltages that reduces a buffer solution and raises the ph in proximity to the electrodes. at higher ph levels double-stranded oligonucleotides de-hybridize. nicks between oligonucleotide complexes and oligonucleotides anchored to the microelectrode array are closed creating covalent attachments. de-hybridized single-stranded oligonucleotides are removed leaving only the oligonucleotides connected to microelectrode array. thus, during a given round of synthesis, oligonucleotide complexes are added only to the locations on the microelectrode array where the electrodes are not activated.