METHOD FOR DETECTING MULTIPLE TARGET NUCLEIC ACIDS

Organization Name

MACCURA BIOTECHNOLOGY CO., LTD.

Inventor(s)

Yuhang Zhao of Chengdu, Sichuan CN

Waner Hu of Chengdu, Sichuan CN

Haixin Wang of Chengdu, Sichuan CN

Qiuping Huang of Chengdu, Sichuan CN

METHOD FOR DETECTING MULTIPLE TARGET NUCLEIC ACIDS

This abstract first appeared for US patent application 20250101514 titled 'METHOD FOR DETECTING MULTIPLE TARGET NUCLEIC ACIDS

Original Abstract Submitted

the present invention relates to the field of molecular biology, in particular to a method for detecting multiple target nucleic acids in a single sample container. the present invention particularly relates to a method for multiplex detection based on digital pcr. the method comprises: mixing a primer-probe composition, a sample to be detected and an amplification reagent to obtain a reaction system; placing the reaction system in a condition that allows a nucleic acid polymerase to perform hybridization and extension reaction, so as to obtain a reaction product; and performing signal acquisition on the reaction product. the detection method of the present invention can realize detection of multiple target nucleic acids, is easy to operate, and can save time.