Insitro, Inc. (20250092456). IN SITU SEQUENCING OF RNA TRANSCRIPTS WITH NON-UNIFORM 5' ENDS

IN SITU SEQUENCING OF RNA TRANSCRIPTS WITH NON-UNIFORM 5' ENDS

Organization Name

Insitro, Inc.

Inventor(s)

Cynthia Hao of Saratoga CA US

Max R. Salick of San Francisco CA US

Ci Chu of Palo Alto CA US

IN SITU SEQUENCING OF RNA TRANSCRIPTS WITH NON-UNIFORM 5' ENDS

This abstract first appeared for US patent application 20250092456 titled 'IN SITU SEQUENCING OF RNA TRANSCRIPTS WITH NON-UNIFORM 5' ENDS

Original Abstract Submitted

disclosed herein are methods for performing in situ sequencing of rna transcripts with non-uniform 5′ ends. during reverse transcription (rt) of rna transcripts, rt enzyme is induced to “template-switch” to a separate oligonucleotide provided as the template for the upstream flanking region. this flanking region is grafted onto the beginning of the cdna, enabling padlock probe detection, rolling circle amplification, and fluorescent in situ sequencing. overall, the disclosed method for in situ sequencing can be applicable for analyzing exogenously introduced transcripts (e.g., identifying and determining impact of a perturbation including a crispr perturbation or shrna/sirna/aso perturbation), analyzing naturally occurring transcripts (e.g., measuring gene expression, detecting splicing events), and analyzing modified, naturally occurring transcripts (e.g., detecting mutations or gene edits).