SINGLE MOLECULE MULTI-MOLECULAR TRACE METHODS AND SYSTEMS

Abstract: methods of sequencing by expansion and related improvements to the sequencing of surrogate polymers in a nanopore are described. the surrogate polymer is formed from a template nucleic acid molecule. a surrogate polymer includes multiple units. each unit includes a reporter code portion. the reporter codes correspond to the different nucleotides. surrogate polymers may get stuck in the nanopore. embodiments described herein address these stuck surrogate polymers. in order to allow for multiple reads on the surrogate polymer, a processive consensus technique can be applied. the surrogate polymer may be moved a few units forward and then fewer units backward so that some of the same reporter codes are identified again. this method allows for multiple reads of the same reporter codes. the surrogate polymer eventually passes through the nanopore in the forward direction. periodically, higher clearing voltages may be applied to clear any stuck surrogate polymer in the nanopore.

Inventor(s): Geoffrey Barrall, Eric Takeshi Harada, Jason David Komadina, John Mannion

CPC Classification: G01N33/48721 (Biological material, e.g. blood, urine (, , , take precedence); Haemocytometers (counting blood corpuscules distributed over a surface by scanning the surface ))

Search for rejections for patent application number 20250231172