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20250230431. Spatial Chip, M (GeneMind Biosciences ., .)

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SPATIAL CHIP, METHOD FOR PREPARING SAME, AND USE THEREOF

Abstract: the present application relates to the field of biological technology, and provides a spatial chip, a method for preparing same, and use thereof. the spatial chip comprises a chip substrate comprising a first surface provided with a plurality of isolated single-stranded nucleic acid amplification clusters; each of the single-stranded nucleic acid amplification clusters comprises a plurality of single-stranded nucleic acid molecules with identical nucleotide sequences, and nucleotide sequences of different single-stranded nucleic acid amplification clusters are different; each of the single-stranded nucleic acid amplification clusters has a known physical coordinate set relative to the spatial chip; the single-stranded nucleic acid molecule comprises at least a barcode sequence with a known sequence, and the barcode sequences of each single-stranded nucleic acid amplification clusters are in one-to-one correspondence with the physical coordinate sets.

Inventor(s): Lei LIU, Zhao LI, Guang LI, Zhifeng LIN, Wen WANG, Chunhui CHANG, Chunyou YANG, Naiqian ZHAN, Fang CHEN, Lei SUN

CPC Classification: C12N15/1065 ({Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags})

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