METHODS FOR PREPARING DNA LIBRARIES AND DETECTING RETROVIRAL INTEGRATION SITES

Abstract: the present disclosure belongs to the field of molecular biology, particularly to the technical field of gene analysis and detection, and specifically relates to a method for preparing a dna library and a method for detecting a retroviral integration site. specifically, the method for preparing the dna library comprises the following steps: 1) fragmenting a genomic dna from a retrovirus-infected cell to obtain dna fragments; 2) subjecting the dna fragments to end-repair, a-tailing, and ligation with an adapter to obtain a ligation product, wherein the adapter is an asymmetric double-strand adapter comprising a long-strand sequence and a short-strand sequence, wherein the long-strand sequence sequentially comprises, from a 5′ end to a 3′ end, a fixed sequence, a random umi sequence, and an amplification primer binding sequence, and the short-strand sequence comprises a sequence complementary to the fixed sequence; and other steps. the detection method of the present disclosure has extremely high sensitivity, thus having good application potential.

Inventor(s): Jin LIU, Qiansheng CAO, Qingqing CHEN, Min ZHANG

CPC Classification: C12N15/1065 ({Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags})

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