COMPOSITIONS AND METHODS FOR MAKING AND USING PROTEIN NANOWIRES WITH TUNABLE FUNCTIONALITY

Abstract: engineered, electrically conductive fimbrial polypeptides, pilus, and bundled pili are provided. the polypeptides typically include one or more mutations (e.g., substitution or addition) with an aromatic amino acid relative to the corresponding wildtype fimbrial protein. in some embodiments, the amino acid is a substrate for a “click” chemistry reaction such as copper(i)-catalyzed azide-alkyne cycloaddition (cuaac), which can be used to conjugate a functional moiety to the non-standard aromatic amino acid residues. preferred functional moieties include conductive materials such as metal (e.g., gold) particles, optionally nanoparticles, and heme groups. also provided are pilus formed of a plurality of the engineered fimbrial polypeptides, and bundles of pili formed of a plurality of the pili. electrical circuits, devices, and systems including the engineered materials, wherein the engineered material serves as the conductive element, and method of use thereof are also provided. exemplary devices include, but are not limited to, sensors, transistors, and capacitors.

Inventor(s): Farren Isaacs, Nikhil Malvankar, Daniel Mark Shapiro, Sibel Ebru Yalcin, Gunasheil Mandava

CPC Classification: C07K14/245 (PEPTIDES (peptides in foodstuffs ; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs ; preparations for medicinal purposes ; peptides containing beta-lactam rings ; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, ; ergot alkaloids of the cyclic peptide type ; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids ; macromolecular products derived from proteins ; preparation of glue or gelatine ; single cell proteins, enzymes ; genetic engineering processes for obtaining peptides ; compositions for measuring or testing processes involving enzymes ; investigation or analysis of biological material ))

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