METHODS FOR CHARACTERISING EXTRACELLULAR VESICLES BY FLUORESCENCE MICROSCOPY, AND METHODS OF IMMOBILISING EXTRACELLULAR VESICLES

Abstract: the present application discloses methods for characterising vesicles. the method involves (1) a sample preparation step, comprising providing a test specimen with vesicles attached to a substrate, wherein the vesicles are labelled with one or more fluorescent probes; (2) an image acquisition step, comprising imaging said one or more fluorescent probes on the vesicles to generate image data; (3) an image processing step which identifies individual vesicles and constructs a feature vector containing characterising parameters for individual vesicles characterising parameters (including a morphological parameter) (4) a data transformation step to calculate modified feature vectors of lower dimensionality for individual vesicles; and (5) a characterisation step, which characterises the vesicles based on the modified feature vectors. the application also discloses methods for immobilising vesicles on a substrate, as well as substrates functionalised to capture vesicles.

Inventor(s): Bo Jing, James Hannes Felce, Andras Gabor Miklosi

CPC Classification: G01N33/582 ({with fluorescent label})

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