METHOD OF USING OF A FUNCTIONALLY CLOSED CELL PROCESSING SYSTEM

Abstract: a method for enhancing genetic modification of target cells through automated spinoculation within a functionally closed cell processing platform. the process begins with introducing a cell suspension into a tapered cell processing cassette (cpc) that facilitates sedimentation and concentration of target cells. genetic modification is achieved by injecting a gene transfer vector into the cpc, followed by controlled spinoculation to enhance vector-cell interaction through centrifugal force while maintaining optimal temperature and conditions. optical sensors and automated feedback systems monitor and adjust centrifugation parameters to maximize transduction efficiency and cell viability. the method includes precise mixing, reagent delivery, washing to remove contaminants, and harvesting of genetically modified cells, all within a sterile environment. additional features include buoyant microbubble separation, multi-mode centrifugation, and automated fluid handling via transfer syringe cassettes, ensuring efficient, contamination-free cell processing suitable for therapeutic applications.

Inventor(s): Philip H. Coelho, Paul Getchel, Jonathan Ellis, Duncan MacGregor, Joël Bodenmann

CPC Classification: C12N15/85 (for animal cells)

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