BIO-ENGINEERED ENZYMES AND USES THEREOF

Abstract: engineered enzymes for amplification of nucleic acid sequences which function at constant temperatures thereby eliminating heating and cooling cycles associated with traditional polymerase chain reaction (pcr) are disclosed. specifically, novel, genetically engineered pcra helicase enzymes are utilized in conjunction with amplification components to provide a method for amplification of nucleic acid sequence by incubating at a substantially isothermal temperature i) a composition comprising a target sequence, ii) primers and iii) said engineered pcra helicase, a single stranded binding protein (ssb), a polymerase, and/or a thermostable pyrophosphatase (ppase) buffer, or combinations thereof.

Inventor(s): Taekjip Ha, Momcilo Gavrilov

CPC Classification: C12N9/90 (MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media ))

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