METHODS AND COMPOSITIONS FOR MEASURING AND INHIBITING EXONUCLEASE ACTIVITY

Abstract: the present disclosure relates to novel methods and compositions for a measuring exonuclease enzyme activity. in particular, the methods entail contacting a sample with a fluorescently-labeled substrate to create a test mixture, incubating the test mixture for a time sufficient for cleavage of the substrate, and measuring the fluorescence signal from the test reaction mixture. in certain aspects, the methods disclosed herein are useful for identifying and/or assessing a modulator of an exonuclease that may be used either alone or in combination with other compounds. the present disclosure also relates to fluorescently-labeled double-stranded nucleic acid compositions useful in the practice of such methods and, still further, to kits for performing the methods of the disclosure. the present disclosure further relates to compounds and methods useful for treating a viral infection.

Inventor(s): Michele PAGANO, Gergely RONA, Andras ZEKE, Bearach MIWATANI-MINTER

CPC Classification: A61K31/519 (PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms ; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles ; soap compositions ))

Search for rejections for patent application number 20250213569