NUCLEIC ACID AMPLIFICATION METHOD, NUCLEIC ACID AMPLIFICATION SYSTEM, VIRUS DETECTION METHOD, AND VIRUS DETECTION SYSTEM

Abstract: to achieve a nucleic acid amplification method and a nucleic acid amplification system capable of simply amplifying a nucleic acid in a short period of time, and a virus detection method and a virus detection system. the nucleic acid amplification method includes: a first preparation step of preparing a first liquid by adding a nonionic surfactant, dntps, and ntps to a specimen containing a virus; a first incubation step of retaining the first liquid in a first temperature zone for a first period of time; a second preparation step of preparing a second liquid by adding a first primer corresponding to a first region of a nucleic acid molecule derived from the virus, a second primer corresponding to a second region, and an enzyme to the first liquid after the first incubation step; and a second incubation step of retaining the second liquid in a second temperature zone for a second period of time.

Inventor(s): Masaki SATO, Kensuke KURIHARA

CPC Classification: C12Q1/6865 (Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS])

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