20250207123. Flow Cells Methods (ILLUMINA, .)
FLOW CELLS AND METHODS
Abstract: an example of a flow cell includes a substrate having depressions separated by interstitial regions: a polymeric hydrogel positioned within each of the depressions; and a plurality of transposome complexes immobilized within each of the depressions by a biotin-containing linker. in this example, each of the plurality of the transposome complexes is of a single type including a transposon end with a portion of a transferred strand hybridized to a portion of a non-transferred strand, wherein the transferred strand includes a first amplification domain and is free of an index sequence.
Inventor(s): Gianluca Andrea Artioli, Johan Sebastian Basuki, Craig Ciesla, Tyler J. Dill, Louise Jane Fraser, Niall Anthony Gormley, Yuan Heng Trevor Ho, Aathavan Karunakaran, Maria Ines Martins Vitoriano, Jessica Moore, Sebastien Georg Gabriel Ricoult, Vicki S. Thomson, Xavier von Hatten, Jacqueline C. Weir
CPC Classification: C12N15/1065 ({Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags})
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- Patent Applications
- ILLUMINA, INC.
- CPC C12N15/1065
- Gianluca Andrea Artioli of Cambridge GB
- Johan Sebastian Basuki of Singapore SG
- Craig Ciesla of Mountain View CA US
- Tyler J. Dill of San Diego CA US
- Louise Jane Fraser of Great Cambourne GB
- Niall Anthony Gormley of Cambridge GB
- Yuan Heng Trevor Ho of Cambridge GB
- Aathavan Karunakaran of Berkeley CA US
- Maria Ines Martins Vitoriano of Baldock GB
- Jessica Moore of Canvey Island GB
- Sebastien Georg Gabriel Ricoult of Cambridge GB
- Vicki S. Thomson of Cambridge GB
- Xavier von Hatten of Cambridge GB
- Jacqueline C. Weir of Cambridge GB