BIOLOGICAL METHOD FOR DECARBOXYLATION OF CANNABINOIDS DIRECTLY IN PLANT TISSUES

Abstract: in vitro production of acidic cannabinoids and their bioconversion to non-acidic form by microorganisms via a method for producing plant tissues containing one or more decarboxylated cannabinoid(s) by (a) inoculating a temporary liquid immersion culture system containing a sterile liquid culture medium containing at least one cytokinin and/or at least one auxin with sterile plant material from a cultivar; (b) cultivating the plant material in the temporary liquid immersion culture system under conditions suitable for growing plant biomass; (c) inoculating the sterile liquid culture medium with one or more bacterial and/or yeast strain(s), (d) cultivating the plant material in the temporary liquid immersion culture system inoculated by the bacterial or yeast strain(s), and (e) collecting plant tissues. also via using one or more bacterial and/or yeast strain(s) for decarboxylating one or more cannabinoid(s) in plant tissues of cultivars cultivated in a temporary liquid immersion culture system.

Inventor(s): Houssein WEHBI, Sarah-Meryll BUET, Julien SECHET

CPC Classification: A01H4/00 (Plant reproduction by tissue culture techniques {; Tissue culture techniques therefor})

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