C2C9 NUCLEASE-BASED NOVEL GENOME EDITING SYSTEM AND USE THEREOF

Abstract: the present invention relates to the field of biomedicine and discloses a use of a c2c9 nuclease as an rna-guided endonuclease, and a small sized genome editing system. the genome editing system comprises a c2c9 nuclease and/or a nucleic acid encoding the c2c9 nuclease, and a guide rna or a nucleic acid encoding the guide rna. the small sized genome editing system of the present invention can perform gene editing on at least one target sequence in a genome of prokaryotic bacteria and eukaryotic cells. the present invention also discloses a use of the genome editing system and an editing method. by using the genome editing system or method of the present invention, a target gene can be precisely knocked out or cut off, the cutting efficiency is high, and precise editing of the target gene is implemented.

Inventor(s): Quanjiang JI, Weizhong CHEN, Jiacheng MA

CPC Classification: C12N15/111 ({General methods applicable to biologically active non-coding nucleic acids})

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