NICOTINAMIDE ADENINE DINUCLEOTIDE (NAD+) PRECURSORS, NAD PATHWAY SUBSTRATES COMPOSITIONS AND USES THEREOF

Abstract: this disclosure is directed to culture media containing a nicotinamide adenine nucleotide (nad) precursor and/or a nad pathway substrate. this disclosure is also directed to in vitro methods of improving the cell state in an embryo by contacting a mammalian embryo with a culture medium supplemented with a nad precursor and/or a nad pathway substrate. the improvements to the cell state may include increasing the presence of an expanded blastocyst, increasing the attachment rate of the embryo, and/or increasing the derivation of embryonic stem cells from the embryo. in certain embodiments, the media and methods use a nad precursor. a variety of different nad precursors can be used in the media and methods, including nicotinic acid (na) and nicotinamide mononucleotide (nmn).

Inventor(s): Eriona Hysolli, Mariana Ianello Giassetti

CPC Classification: C12N5/0603 (MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media ))

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