PREPARATION METHOD AND APPLICATION OF SINGLE-CHAIN ANTIBODY FRAGMENT TARGETING SARS-COV-2 NUCLEOCAPSID PROTEIN

Abstract: disclosed are a preparation method and application of a single-chain antibody fragment targeting sars-cov-2 nucleocapsid protein, aiming to provide a single-chain antibody fragment with high affinity and strong specificity. the present disclosure obtains three single-chain antibody fragments targeting sars-cov-2 nucleocapsid protein through construction of a nanobody phage library and phage screening technology. the single-chain antibody fragment consists of a heavy chain variable region, a 15aa connecting peptide, and a light chain variable region connected in sequence, and has an amino acid sequence shown in seq id no: 1-seq id no: 3. the single-chain antibody fragment in the present disclosure has excellent binding performance and specificity with the sars-cov-2 nucleocapsid protein, can be applied to a variety of immunoassay platforms of the sars-cov-2, and has broad application prospects in the field of sars-cov-2 detection.

Inventor(s): Yi Luo, Wenjin Hu, Shixiang Yang, Xiaolong Wang, Shengyang Wang, Huai Lin, Xiang Long

CPC Classification: C07K16/1003 (PEPTIDES (peptides in foodstuffs ; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs ; preparations for medicinal purposes ; peptides containing beta-lactam rings ; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, ; ergot alkaloids of the cyclic peptide type ; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids ; macromolecular products derived from proteins ; preparation of glue or gelatine ; single cell proteins, enzymes ; genetic engineering processes for obtaining peptides ; compositions for measuring or testing processes involving enzymes ; investigation or analysis of biological material ))

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