TRANSIENT EXPRESSION OF THERAPEUTIC PROTEINS

Abstract: the present disclosure provide novel methods of large-scale production of recombinant proteins, e.g., therapeutic proteins such as antibodies, comprising concentrating an eukaryotic cell culture to a high density and transiently transfecting the eukaryotic cells with a polynucleotide encoding the recombinant protein using electroporation, e.g., flow electroporation. in some aspects, the culture is performed under perfusion conditions using, e.g., a tangential flow filtration method such as alternating tangential flow filtration. the proteins obtained using the disclosed methods are comparable to those produced using stable transfection. the methods disclosed herein can be used, for example, to accelerate therapeutic agent development, to reduce host cell toxicity, or for individualized therapeutics such as small scale manufacturing of treatments for rare or orphan diseases.

Inventor(s): Kitty AGARWAL, Juan Camilo GONZALEZ RIVERA, Todd William RYDER, Anurag KHETAN

CPC Classification: C07K16/00 (Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies {(antibodies with enzymatic activity, e.g. abzymes )})

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