ULTRA-SENSITIVE DETECTION OF MOLECULES OR PARTICLES USING BEADS OR OTHER CAPTURE OBJECTS

Abstract: the present invention relates to systems and methods for detecting analyte molecules or particles in a fluid sample and in some cases, determining a measure of the concentration of the molecules or particles in the fluid sample. methods of the present invention may comprise immobilizing a plurality of analyte molecules or particles with respect to a plurality of capture objects. at least a portion of the plurality of capture objects may be spatially separated into a plurality of locations. a measure of the concentration of analyte molecules in a fluid sample may be determined, at least in part, on the number of reaction vessels comprising an analyte molecule immobilized with respect to a capture object. in some cases, the assay may additionally comprise steps including binding ligands, precursor labeling agents, and/or enzymatic components.

Inventor(s): David C. Duffy, David M. Rissin, David R. Walt, David Fournier, Cheuk Kan

CPC Classification: G01N33/54393 ({Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding})

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