A TUNABLE PROXIMITY ASSAY THAT CAN OVERCOME DILUTIONAL NON-LINEARITY

Abstract: provided herein are methods of tuning a proximity reporter assay. the methods include providing multiple series of compositions with first binding agents labeled with nucleic acid molecules, wherein compositions in the series comprise the first and second binding agents at different concentrations from the other compositions in the series, forming a plurality of mixtures by contacting each composition from the series of compositions with the target analytes; performing, for each mixture in the plurality of mixtures, a proximity assay, wherein the assay produces, for each mixture, a signal comprising a nucleic acid product and selecting from each of the series of compositions a single composition comprising binding agents at a concentration that results in signals within four orders of magnitude for the target analytes. the method can be performed for any number of analytes.

Inventor(s): Hyongsok Soh, Brandon D. Wilson, Sharon Shin Newman, Daniel Mamerow

CPC Classification: G01N33/5306 ({Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding})

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