MICROORGANISM STRAIN FOR ANTIBIOTIC-FREE PLASMID-BASED FERMENTATION AND METHOD FOR GENERATION THEREOF

Abstract: the present invention relates to means for recombinant manufacture. in particular, it relates to a recombinant bacterial cell comprising in its genome at least one essential gene the endogenous promoter of which has been replaced by a heterologous expression control sequence being inducible by an inducer molecule, such that the expression of said essential gene in the recombinant bacterial cell is dependent on the presence of said inducer molecule. further contemplated are methods for generating the recombinant bacterial cell of the invention as well as a method for recombinant manufacture of a compound of interest. the present invention also provides for use the recombinant bacterial cell of the invention for recombinant manufacture of a compound of interest and a kit for manufacture of a compound of interest.

Inventor(s): Harald KRANZ, Marlen SCHMIDT, Katherine Emily BRECHUN, Marion FOERSCHLE

CPC Classification: C12N15/635 ({Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline})

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