DNA Methylation Library Construction Method and Library Obtained with Same, DNA Hybridization and Capture Method and Kits

Abstract: a dna methylation library construction method and a library obtained with same, a dna hybridization and capture method and kits. the construction method comprises: providing a test sample comprising a plurality of double-stranded dna fragments; denaturing the double-stranded dna fragments into single-stranded dna; ligating the single-stranded dna and a double-stranded adapter sequence to obtain single-stranded dna linked with the double-stranded adapter sequence; extending the single-stranded dna linked with the double-stranded adapter sequence to form an extended fragment being linked with the double-stranded adapter sequence of the single-stranded dna; oxidating methylated cytosine in double-stranded dna linked with the double-stranded adapter sequence to obtain double-stranded dna having protected cytosine; removing the extended fragment to obtain single-stranded dna having protected cytosine; deaminating unmethylated cytosine in the single-stranded dna having the protected cytosine to obtain single-stranded dna having uracil; and amplifying the single-stranded dna having uracil to obtain a dna methylation library.

Inventor(s): Bangquan YE

CPC Classification: C12N15/1093 ({General methods of preparing gene libraries, not provided for in other subgroups})

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