Automated Isolation of Viable Mitochondria

Abstract: an automated isolation device for isolation of mitochondria includes an incubation station including a holder for a viable mitochondria solution; and a cooling system, controlled by a processor of the device, for cooling the holder. the device includes a processor-controlled transfer system for transferring solution from the holder to a filtration station; and the filtration station, including a series of filters. the device includes a processor-controlled spectrometry station including a spectrometer positioned to illuminate a cuvette fluidically coupled to an output of the filtration station; and a detector coupled to the processor and positioned on a side of the cuvette opposite the spectrometer. the device includes a processor-controlled transfer system for transferring solution from the spectrometry station to a centrifuge. the centrifuge is processor-controlled and configured to centrifuge the filtrate to separate viable mitochondria from a supernatant.

Inventor(s): James D. McCully, Douglas B. Cowan, Pedro J. del Nido, Thomas Duignan

CPC Classification: C12M41/48 ({Automatic or computerized control (automatic analysis )})

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