METHODS FOR THE SELECTIVE REMOVAL OF CONTAMINANTS DURING A PROCESS OF PURIFYING BIOLOGIC AGENTS

Abstract: the present invention relates to methods for the selective removal of contaminants during a process of purifying a biologic agent, comprising a step of subjecting a solution containing (i) said biologic agent and (ii) potential contaminants to a filtration step using a charge-neutral organic polymer filter in the presence of a kosmotropic agent, wherein said one or more contaminants are contaminants that bind to the charge-neutral organic polymer filter in the presence of said at least one kosmotropic agent, while the biologic agent does not bind to the charge-neutral organic polymer filter in the presence of said at least one kosmotropic agent. these methods combine a dual use of the filter for chemical and mechanical removal of contaminants in a single step. the present invention further relates to respective methods for the purification of a biologic agent, as well as respective uses of a charge-neutral organic polymer filter.

Inventor(s): Klemen Bozic, Rok Sekirnik, Urh Cernigoj

CPC Classification: C07K1/34 (PEPTIDES (peptides in foodstuffs ; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs ; preparations for medicinal purposes ; peptides containing beta-lactam rings ; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, ; ergot alkaloids of the cyclic peptide type ; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids ; macromolecular products derived from proteins ; preparation of glue or gelatine ; single cell proteins, enzymes ; genetic engineering processes for obtaining peptides ; compositions for measuring or testing processes involving enzymes ; investigation or analysis of biological material ))

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