RECOMBINANT ADENOVIRUS VACCINE FOR AFRICAN SWINE FEVER AND METHOD FOR CONSTRUCTING SAME

Abstract: an african swine fever virus vaccine includes five groups of antigens in total, and each group is respectively obtained by constructing recombinant adenovirus vectors co-expressing four antigen genes of african swine fever virus, and packaged by 293td37 cells. the four antigenic genes of african swine fever virus in each group are 1, p72, b602l, p30 and p54; 2, cp129rubiqutin, mgf5l6l, cp312r, and mgf110-4l; 3, l8lubiqutin, i215l, i73rhbsaghbsag and e146l; 4, ep402r, ep153r, i177l, and k205rubiqutin; 5, f317l, a151r, p34, and pp62. the construction of the recombinant adenovirus vector for co-expression of four antigen genes of the african swine fever virus mainly includes: knocking out e1, e3, e2a and e4 genes of the adenovirus vector by crispr/cas9 technology, constructing an orf6/7 expression frame of e4 in an e2a region, and constructing shuttle plasmids in e1 and e4 regions for appropriately expressing four antigen genes, thereby obtaining a completely new adenovirus vector.

Inventor(s): Ping CHEN, Tingting ZHANG, Na LI, Xintao ZHONG, Zhigang ZHU, Nan LI

CPC Classification: A61K39/12 (PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms ; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles ; soap compositions ))

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