METHOD FOR DETERMINING CHEMICAL STRUCTURE OF LIPID AND ION MOBILITY-TANDEM MASS SPECTROMETER

Abstract: the present disclosure relates to the field of mass spectrometry, and particularly provides a method for determining a chemical structure of a lipid and an ion mobility-tandem mass spectrometer. the method for determining a chemical structure of a lipid includes: an ionization step of ionizing a sample to obtain sample ions; an ion mobility-based separation step of separating target lipid ions from the sample ions based on ion mobility; a first dissociation step of dissociating the target lipid ions with dissociation energy adjusted to break a first chemical bond of the target lipid ions; a mass-based selection step of selecting the target lipid ions, whose first chemical bond is broken, based on a mass number to obtain fragment ions; a second dissociation step of dissociating the fragment ions to at least break a second chemical bond of the fragment ions which has bond energy higher than the first chemical bond, to obtain diagnostic ions; and a mass analysis step of performing a mass analysis on the diagnostic ions.

Inventor(s): Wenjian SUN, Yuling Li, Xiaoqiang ZHANG, Kang GUO

CPC Classification: G01N33/92 (involving lipids, e.g. cholesterol {, lipoproteins, or their receptors (steroid hormones )})

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