20250171769. Spatial Transposition-based Rna (Illumina, .)
SPATIAL TRANSPOSITION-BASED RNA SEQUENCING LIBRARY PREPARATION METHOD
Abstract: an rna sequencing library preparation process that that utilized transposition with or with a template switch oligonucleotide to generate the libraries having umis and spatial barcode information, and methods for improving rna library preparation from tissue samples using template switching and thermal amplification to improve rna library quality.
Inventor(s): Lena Storms, Craig April, Mats Ekstrand, Kerou Zhang, Yao Xiao, Brittany Flowers, Anustup Poddar, Andrea Manzo, Olivia Ghazinejad, Fei Shen, Brian Mather, Se Min Canon, Andrew Ostrow
CPC Classification: C12N15/1065 ({Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags})
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- Patent Applications
- Illumina, Inc.
- CPC C12N15/1065
- Lena Storms of San Diego CA US
- Craig April of San Diego CA US
- Mats Ekstrand of San Diego CA US
- Kerou Zhang of San Diego CA US
- Yao Xiao of San Diego CA US
- Brittany Flowers of San Diego CA US
- Anustup Poddar of San Diego CA US
- Andrea Manzo of San Diego CA US
- Olivia Ghazinejad of San Diego CA US
- Fei Shen of San Diego CA US
- Brian Mather of San Diego CA US
- Se Min Canon of Philadelphia PA US
- Andrew Ostrow of San Diego CA US