METHODS OF PRODUCING RECOMBINANT PROTEINS

Abstract: in certain embodiments, this disclosure provides novel methods of increasing the viable cell density of an n-1 large-scale bioreactor cell culture, comprising culturing a host cell expressing a recombinant poly peptide of interest in a non-perfusion-based culture system, and wherein the viable cell density is increased to at least 5�10cells/ml. in certain embodiments, the disclosure provides novel methods for large-scale production of a recombinant polypeptide of interest, comprising: (1) culturing a host cell expressing a recombinant polypeptide of interest in an n-1 stage in a non-perfusion-based culture system, wherein the viable cell density is increased to at least 5�10cells/ml: and (2) culturing n fed-batch production cells in an enriched media with high-seed density at least 1.5�10cells/ml, wherein the n fed-batch production cells are inoculated from the n-1 stage in a non-perfusion-based culture system.

Inventor(s): Jianlin XU, Andrew YONGKY, Jun TIAN, Michael C. BORYS, Zhengjian LU

CPC Classification: C12N5/0018 (Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor; (plant reproduction by tissue culture techniques ))

Search for rejections for patent application number 20250171732