GLYCOSYL HYDROLASE ENZYMES IN HIGH TEMPERATURE INDUSTRIAL PROCESSES

Abstract: novel hyperthermophilic beta-mannanases are provided for use in high temperature industrial applications requiring enzymatic hydrolysis of 1,4-�-d-mannosidic linkages in mannans, galactomannans, and glucomannans. also provided are methods and compositions for fracturing a subterranean formation in which a gellable fracturing fluid is first formed by blending together a hydratable polymer and a beta-mannanase as an enzyme breaker. an optimized and stabilized recombinant beta-mannanase is provided that shows superior performance/effectiveness and properties in degrading guar and derivatized guars at ph ranges from 3.0 to 12 and temperatures ranging from 130� f. to in excess of 270� f.

Inventor(s): Robert Muko Tjon-Joe-Pin, Moreland David Gibbs, Valentino Setoa Junior Te'o

CPC Classification: C09K8/68 (containing organic compounds)

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