SYSTEMS AND METHODS FOR ON-CHIP ANALYSIS OF NUCLEIC ACIDS AND FOR MULTIPLEXED ANALYSIS OF CELLS

Abstract: a method for detecting a nucleic acid target, the method comprising: introducing a sample comprising a cell into a microfluidic system comprising an input component for the introduction of the sample, a microfluidic chip detachably disposed within the system and connected to the input component, a collection reservoir connected to an output port of the microfluidic chip, and a detection component; and operating the system to flow the sample through the chip and (i) capture the cell within the chip; (ii) extract nucleic acid from the captured cell; and (iii) capture the nucleic acid at nucleic acid entanglement micropillars within the channel.

Inventor(s): Harold G. CRAIGHEAD, Harvey C. TIAN, David M. LIN, Adam J. BISOGNI

CPC Classification: B01L3/502761 ({specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules (investigating characteristics of particles )})

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