ON-SLIDE SYNTHESIS OF A SCHIFF REAGENT

Abstract: the present disclosure is directed to compositions, kits, and methods for synthesizing a schiff's reagent in situ, i.e., directly on a substrate, such as within a puddle disposed on the surface of a substrate. in some embodiments, the kits comprise at least a dye composition and a sulfite composition. in some embodiments, the dye composition comprises a compound having a 4-benzhydrylidene-2,5-cyclohexadien-1-imine core functionalized to include at least two substituted or unsubstituted amine groups, wherein the compound has a molecular weight ranging from between about 300 g/mol to about 600 g/mol. in some embodiments, the dye composition optionally includes an acid. in some embodiments, the sulfite composition comprises a sulfite or sosource.

Inventor(s): Jean Marie Bird, Mary Jo Castro Cantoria, Edward E. Durrant, Andrew Hannon

CPC Classification: G01N1/30 (INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing processes other than immunoassay, involving enzymes or microorganisms , ))

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