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20250163503. Method Performing (Gen-Probe Incorporated)

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METHOD OF PERFORMING A LAB DEVELOPED TEST

Abstract: performing multiple nucleic acid amplification assays in an automated analyzer comprises loading the analyzer with a sample-containing receptacles, producing a purified form of a first sample to isolate a first analyte, producing a purified form of a second sample to isolate a second analyte, forming first and second amplification reaction mixtures with the purified first and second samples, the first mixture containing first amplification oligomers for amplifying a first region of the first analyte and the second mixture containing second amplification oligomers for amplifying a second region of the second analyte, exposing the second mixture to thermal conditions for amplifying the second region, exposing the first mixture to thermal conditions for amplifying the first region, determining the presence or absence of the second analyte in the second mixture, and determining the presence or absence of the first analyte in the first mixture.

Inventor(s): Jennifer L. TIDD, Anne-Laure SHAPIRO, Ankur H. SHAH, James T. TUGGLE, David OPALSKY, Alberto A. LIO, Timothy J. SCHEER, Jason F. RHUBOTTOM, David Aaron BUSE

CPC Classification: C12Q1/6844 (Nucleic acid amplification reactions)

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