ENZYMATIC SYNTHESIS OF POLYNUCLEOTIDE

Abstract: provided is a method and a kit for synthesizing and retrieving a polynucleotide enzymatically, including an initiator containing having a free 3′-hydroxyl group; a polymerase for incorporating nucleotide monomers to the initiator to form a nucleic acid strand from the free 3′-hydroxyl group, wherein the nucleic acid strand includes a guiding nucleotide to be recognized by an endonuclease; and the endonuclease cleaving the nucleic acid strand to release a predetermine polynucleotide and newly form a free 3′-hydroxyl group at 3′ end of the remaining nucleic acid strand which serve as a reusable initiator. also provided is a kit for synthesizing and retrieving a polynucleotide using the method.

Inventor(s): CHENG-YAO CHEN, YI-WEN CHENG, TSU-YING WU, WEN-TING CHEN

CPC Classification: C12P19/34 (FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer ; producing vinegar ; producing specific peptides or proteins ; producing enzymes ; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification ; measuring or testing processes involving enzymes or microorganisms ; measuring or testing processes involving nucleic acid amplification reactions ; fermentation processes to form a food composition, or ; compounds in general, see the relevant compound class, e.g. , )})

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