Nucleic Acid Strand Cleaving Method, Nucleic Acid Strand Cleaving Device, Double-Stranded DNA Production Method, and Double-Stranded DNA Production Device

Abstract: there is provided a nucleic acid strand cleaving method including a nucleic acid preparation step of preparing a nucleic acid to be cleaved having a structure represented by the following formula (1), and a cleaving step of reacting the nucleic acid to be cleaved with a cleaving agent to cleave the nucleic acid to be cleaved at the part x of the formula (1) to generate a nucleic acid having a structure represented by the following formula (2). the cleaving agent is a metal nanoparticle containing an atom selected from the group consisting of silver, mercury, and cadmium. [chemical formula 1] here, b represents a base, and x represents sulfur or selenium. nuca is composed of at least one nucleotide and is a part of the nucleic acid to be cleaved, and represents a part on the 5′ end side with reference to the x. nucb is composed of at least one nucleotide and is a part of the nucleic acid to be cleaved, and represents a part on the 3′ end side with reference to the x.

Inventor(s): Hiroshi Abe, Masahito Inagaki, Fumitaka Hashiya, Haruka Hiraoka, Naoko Abe

CPC Classification: C07H21/04 (SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS (derivatives of aldonic or saccharic acids , ; aldonic acids, saccharic acids , ; cyanohydrins ; glycals ; compounds of unknown constitution ; polysaccharides, derivatives thereof ; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification ; sugar industry ))

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