METHOD AND MEANS FOR AN ISOLATION OF MEMBRANE-BOUND PROTEINS FROM A BIOLOGICAL SAMPLE

Abstract: the present invention provides the method and means for an efficient isolation of membrane-bound proteins from biological samples, e.g. in samples from raw or processed plant material. the biological sample can be highly processed, e.g. by applying high temperature, pressure, or a chemical treatment and can be derived from seed matrices as well as other typical plant tissues for example seed, grain, leaf, root, or pollen. the invention comprises the provision of a novel extraction buffer (meb) and its application in the method of the invention.

Inventor(s): Meghan K. Wyrick, Min Xia, Janna Slessareva

CPC Classification: C07K1/145 (Extraction; Separation; Purification)

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