18983773. LINKER STRUCTURES WITH MINIMAL SCAR FOR ENZYMATIC SYNTHESIS (MICROSOFT TECHNOLOGY LICENSING, LLC)

LINKER STRUCTURES WITH MINIMAL SCAR FOR ENZYMATIC SYNTHESIS

Organization Name

MICROSOFT TECHNOLOGY LICENSING, LLC

Inventor(s)

Bichlien Hoang Nguyen of Seattle WA US

Jake Smith of Seattle WA US

LINKER STRUCTURES WITH MINIMAL SCAR FOR ENZYMATIC SYNTHESIS

This abstract first appeared for US patent application 18983773 titled 'LINKER STRUCTURES WITH MINIMAL SCAR FOR ENZYMATIC SYNTHESIS

Original Abstract Submitted

This disclosure provides electrochemically-cleavable linkers with cleavage potentials that are less than the redox potential of the solvent in which the linkers are used. In some applications, the solvent may be water or an aqueous buffer solution. The linkers may be used to link a nucleotide to a bound group. The linkers include a cleavable group which may be one of a methoxybenzyl alcohol, an ester, a propargyl thioether, or a trichloroethyl ether. The linkers may be cleaved in solvent by generating an electrode potential that is less than the redox potential of the solvent. In some implementations, an electrode array may be used to generate localized electrode potentials which selectively cleave linkers bound to the activated electrode. Uses for the linkers include attachment of blocking groups to nucleotides in enzymatic oligonucleotide synthesis.