18808889. SYSTEMS AND DEVICES FOR SIGNAL CORRECTIONS IN SEQUENCING (ILLUMINA, INC.)
SYSTEMS AND DEVICES FOR SIGNAL CORRECTIONS IN SEQUENCING
Organization Name
Inventor(s)
Emrah Kostem of Menlo Park CA US
SYSTEMS AND DEVICES FOR SIGNAL CORRECTIONS IN SEQUENCING
This abstract first appeared for US patent application 18808889 titled 'SYSTEMS AND DEVICES FOR SIGNAL CORRECTIONS IN SEQUENCING
Original Abstract Submitted
The technology disclosed relates to determining tag signals from measured intensities for purposes of base calling in next-generation sequencing. In particular, the measured intensities are collected by light sensors in a sensor array directed to a sample surface including pixel areas and holding a plurality of clusters during a sequence of sampling events. Each light sensor is directed to and measuring intensity from one of the pixel areas during each sampling event. The method includes adjusting the measured intensities from a pixel in the pixel areas for background intensity based on variations in background levels of the light sensors in the sensor array and determining an intensity of a tag signal originating from the pixel based on the adjusted measured intensities of the pixel.
