18763564. HIGH-EFFICIENCY EXPRESSION VECTOR OF CIRCULAR RIBONUCLEIC ACID (circRNA) AND USE THEREOF (ZHEJIANG UNIVERSITY)
HIGH-EFFICIENCY EXPRESSION VECTOR OF CIRCULAR RIBONUCLEIC ACID (circRNA) AND USE THEREOF
Organization Name
Inventor(s)
Jiyong Zhou of Hangzhou City (CN)
Liuyang Du of Hangzhou City (CN)
Jinyan Gu of Hangzhou City (CN)
Yulan Jin of Hangzhou City (CN)
Weiren Dong of Hangzhou City (CN)
HIGH-EFFICIENCY EXPRESSION VECTOR OF CIRCULAR RIBONUCLEIC ACID (circRNA) AND USE THEREOF
This abstract first appeared for US patent application 18763564 titled 'HIGH-EFFICIENCY EXPRESSION VECTOR OF CIRCULAR RIBONUCLEIC ACID (circRNA) AND USE THEREOF
Original Abstract Submitted
Provided is a high-efficiency expression vector of a circular ribonucleic acid (circRNA). The high-efficiency expression vector is an expression framework with a total length of only 173 bp and having a nucleotide sequence shown in SEQ ID NO: 1, and includes an upstream frame sequence of 92 bp, a downstream frame sequence of 75 bp, and a restriction site EcoR I located between the upstream and downstream frame sequences for inserting a gene to be circularized. In the present disclosure, the high-efficiency expression vector of a circRNA can enable an exogenously-inserted sequence to efficiently express the circRNA, thus providing an efficient expression tool for obtaining the circRNA. On the premise of ensuring efficient circularization of the circRNA, lengths of upstream and downstream flanking sequences required for the circularization have been highly optimized to further reduce the complexity of splicing by-products caused by the flanking sequences.
