18709939. MODULATION OF THE CRISPR ROADBLOCK (CORNELL UNIVERSITY)
MODULATION OF THE CRISPR ROADBLOCK
Organization Name
Inventor(s)
Michelle D. Wang of Ithaca NY (US)
Porter M. Hall of Ithaca NY (US)
MODULATION OF THE CRISPR ROADBLOCK
This abstract first appeared for US patent application 18709939 titled 'MODULATION OF THE CRISPR ROADBLOCK
Original Abstract Submitted
Provided are modified guide RNAs (gRNAs) for use with CRISPR Cas proteins. A modified guide RNA comprises at its 5′ or 3′end at least 5 nucleotides that comprise an inverted repeat sequence having a segment targeted to a spacer sequence in DNA. The inverted repeat sequence is configured so that it can concurrently be hybridized to the spacer sequence and to the complementary strand of the DNA comprising the spacer sequence when in the presence of the DNA and the Cas protein. The modified gRNA influences the Cas protein interaction with DNA.