SINGLE-MOLECULE PROTEIN AND PEPTIDE SEQUENCING

Organization Name

Massachusetts Institute of Technology

Inventor(s)

Daniel Masao Estandian of Cambridge MA (US)

Alexi Georges Choueiri of Chandler AZ (US)

Edward Stuart Boyden of Chestnut Hill MA (US)

Asmamaw Wassie of Boston MA (US)

SINGLE-MOLECULE PROTEIN AND PEPTIDE SEQUENCING - A simplified explanation of the abstract

This abstract first appeared for US patent application 18615276 titled 'SINGLE-MOLECULE PROTEIN AND PEPTIDE SEQUENCING

The present description provides methods, assays, and reagents useful for sequencing proteins. Sequencing proteins involves observing the identity and order of amino acids, useful for sequencing single polypeptide molecules or multiple molecules of a single polypeptide. The methods are also useful for sequencing multiple polypeptides, enabling high resolution interrogation of the proteome and ultrasensitive diagnostics for early disease detection.

Useful methods, assays, and reagents for sequencing proteins
Can sequence single polypeptide molecules or multiple molecules of a single polypeptide
Useful for sequencing multiple polypeptides
Enables high resolution interrogation of the proteome
Enables ultrasensitive diagnostics critical for early disease detection

Potential Applications: - Biomedical research - Clinical diagnostics - Drug development

Problems Solved: - Lack of high resolution protein sequencing methods - Inability to detect diseases at an early stage

Benefits: - Improved understanding of protein structures - Early detection of diseases - Potential for personalized medicine

Commercial Applications: Title: Protein Sequencing Technology for Biomedical and Diagnostic Applications This technology can be used in biomedical research, clinical diagnostics, and drug development, providing high-resolution protein sequencing for improved disease detection and personalized medicine.

Questions about Protein Sequencing Technology: 1. How does protein sequencing technology improve disease detection? Protein sequencing technology enables the high-resolution interrogation of the proteome, allowing for the early detection of diseases by identifying specific protein sequences associated with various conditions.

2. What are the potential commercial uses of protein sequencing technology? Protein sequencing technology has applications in biomedical research, clinical diagnostics, and drug development, offering improved understanding of protein structures and enabling personalized medicine.

Original Abstract Submitted

The present description provides methods, assays and reagents useful for sequencing proteins. Sequencing proteins in a broad sense involves observing the plausible identity and order of amino acids, which is useful for sequencing single polypeptide molecules or multiple molecules of a single polypeptide. In one aspect, the methods are useful for sequencing multiple polypeptides. The methods and reagents described herein can be useful for high resolution interrogation of the proteome and enabling ultrasensitive diagnostics critical for early detection of diseases.