18582764. METHOD AND KIT FOR GENETIC ANALYSIS (Hitachi, Ltd.)
METHOD AND KIT FOR GENETIC ANALYSIS
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METHOD AND KIT FOR GENETIC ANALYSIS
This abstract first appeared for US patent application 18582764 titled 'METHOD AND KIT FOR GENETIC ANALYSIS
Original Abstract Submitted
A method and kit for genetic analysis can accurately determine whether a plurality of genetic mutations in close proximity are present in a positional relationship of a cis-form or a trans-form and quantify the genetic mutations. The method for genetic analysis includes: preparing probes corresponding to each of a plurality of genetic mutations; performing an amplification reaction using a solution comprising a primer for amplifying a region comprising the plurality of genetic mutations, the probes, a test biological sample, and an enzyme; measuring a binding of an amplicon to the probe by melting curve analysis at varying temperatures of the solution after performing the amplification reaction; and determining, based on a result of the melting curve analysis, whether the plurality of genetic mutations are on a same allele in a cis form or on different alleles in a trans form in DNA contained in the test biological sample.