METHODS OF SEQUENCING USING 3' BLOCKED NUCLEOTIDES

Organization Name

ILLUMINA, INC.

Inventor(s)

Antoine Francais of Cambridge (GB)

Elena Cressina of Cambridge (GB)

Angelica Mariani of Cambridge (GB)

Adam Culley of Cambridge (GB)

Anno Koetje of Cambridge (GB)

Xiaohai Liu of Cambridge (GB)

Xiaolin Wu of Cambridge (GB)

Kathryn Hattingh of Cambridge (GB)

METHODS OF SEQUENCING USING 3' BLOCKED NUCLEOTIDES - A simplified explanation of the abstract

This abstract first appeared for US patent application 18393220 titled 'METHODS OF SEQUENCING USING 3' BLOCKED NUCLEOTIDES

The patent application relates to nucleotide and nucleoside molecules with acetal or allyl 3′ blocking groups, methods to prepare them, and their use in sequencing applications.

Fully functionalized nucleotides with 3′ blocking groups
Nucleotide and nucleoside molecules with acetal or allyl blocking groups
Methods for preparing nucleotide and nucleoside molecules with blocking groups
Use of fully functionalized nucleotides for sequencing applications
Enhancing sequencing accuracy and efficiency

Potential Applications: - Next-generation sequencing technologies - Molecular biology research - Pharmaceutical industry for drug development - Diagnostic applications in healthcare

Problems Solved: - Improving sequencing accuracy by preventing unwanted reactions - Enhancing efficiency in sequencing processes - Facilitating the study of genetic information

Benefits: - Increased accuracy in sequencing results - Streamlined sequencing processes - Enhanced understanding of genetic data - Potential for new drug discoveries and personalized medicine

Commercial Applications: Title: "Innovative Nucleotide Sequencing Technology for Enhanced Accuracy" This technology can be utilized in research institutions, pharmaceutical companies, and diagnostic laboratories for advancing genetic analysis and drug development efforts.

Questions about Nucleotide Sequencing Technology: 1. How does the use of acetal or allyl blocking groups improve sequencing accuracy?

  - The blocking groups prevent unwanted reactions, leading to more precise sequencing results.

2. What are the potential long-term implications of this technology in the field of personalized medicine?

  - This technology could enable more accurate genetic analysis for tailored medical treatments.

Original Abstract Submitted

Embodiments of the present disclosure relate to nucleotide and nucleoside molecules with acetal or allyl 3′ blocking groups. Also provided herein are methods to prepare such nucleotide and nucleoside molecules, and the uses of fully functionalized nucleotides containing the 3′ blocking groups for sequencing applications.