18389877. MULTI-SURFACE BIOLOGICAL SAMPLE IMAGING SYSTEM AND METHOD simplified abstract (ILLUMINA, INC.)

MULTI-SURFACE BIOLOGICAL SAMPLE IMAGING SYSTEM AND METHOD

Organization Name

ILLUMINA, INC.

Inventor(s)

Shaoping Lu of Palo Alto CA (US)

Marco Krumbuegel of San Ramon CA (US)

Stanley Hong of Palo Alto CA (US)

Georg Nadorff of Rochester NY (US)

MULTI-SURFACE BIOLOGICAL SAMPLE IMAGING SYSTEM AND METHOD - A simplified explanation of the abstract

This abstract first appeared for US patent application 18389877 titled 'MULTI-SURFACE BIOLOGICAL SAMPLE IMAGING SYSTEM AND METHOD

• • Simplified Explanation:**

A machine with a lens and flow cell can image biological samples on multiple surfaces of a support structure by capturing light emitted from nucleic acids.

• • Key Features and Innovation:**

- Machine includes a lens, flow cell, and controller for imaging biological samples. - Light emitted from nucleic acids on different surfaces of the flow cell can be captured at varying distances from the lens. - Lens is immersed in a fluid, and surfaces of the flow cell are separated by another fluid. - Machine ensures equal treatment of marginal and axial light rays in the lens's field of view.

• • Potential Applications:**

- Medical diagnostics - Biomedical research - Environmental monitoring

• • Problems Solved:**

- Efficient imaging of biological samples on multiple surfaces - Precise capture of light emitted from nucleic acids - Consistent treatment of light rays in the lens's field of view

• • Benefits:**

- Enhanced imaging capabilities - Improved accuracy in analyzing biological samples - Increased efficiency in research and diagnostics

• • Commercial Applications:**

Potential commercial applications include: - Biotechnology companies - Research laboratories - Medical imaging facilities

• • Questions about the Technology:**

1. How does the machine ensure equal treatment of marginal and axial light rays in the lens's field of view?

  - The machine adjusts the lens at different distances from the flow cell to achieve this balance.

2. What are the specific benefits of using a flow cell in imaging biological samples on multiple surfaces?

  - The flow cell allows for precise separation and analysis of samples on different surfaces.

Original Abstract Submitted

Biological samples on multiple surfaces of a support structure may be imaged using a machine comprising a lens, a flow cell and a controller. Such a machine may capture light emitted from nucleic acids disposed on first and second surfaces of the flow cell when the lens is, respectively, at first and second distances from the flow cell. In such a machine, the lens may be immersed in a first fluid, and the first and second surfaces of the flow cell may be separated by a second fluid. Additionally, in such a machine, differences between marginal and axial light rays in the field of view of the lens may be substantially equal when the lens is at the first and second distances from the flow cell.