Zhejiang University (20240270824). HEMOGLOBIN (Hb) RECOMBINANT EXPRESSION VECTOR AND RECOMBINANT ENGINEERING BACTERIUM, AND PREPARATION METHOD AND USE THEREOF simplified abstract
HEMOGLOBIN (Hb) RECOMBINANT EXPRESSION VECTOR AND RECOMBINANT ENGINEERING BACTERIUM, AND PREPARATION METHOD AND USE THEREOF
Organization Name
Inventor(s)
Wei Chen of Hangzhou City (CN)
Lianghua Xie of Hangzhou City (CN)
Yunhe Meng of Hangzhou City (CN)
Kaihao You of Hangzhou City (CN)
HEMOGLOBIN (Hb) RECOMBINANT EXPRESSION VECTOR AND RECOMBINANT ENGINEERING BACTERIUM, AND PREPARATION METHOD AND USE THEREOF - A simplified explanation of the abstract
This abstract first appeared for US patent application 20240270824 titled 'HEMOGLOBIN (Hb) RECOMBINANT EXPRESSION VECTOR AND RECOMBINANT ENGINEERING BACTERIUM, AND PREPARATION METHOD AND USE THEREOF
Simplified Explanation: The patent application discusses a gene expression cassette encoding hemoglobin (hb) and its use in genetic engineering. It introduces signal peptides that can increase the expression level of leghemoglobin (legh) in a recombinant strain.
- Recombinant strain with signal peptides pel b, pho a, and omp a inserted under the same conditions shows increased expression levels of leghemoglobin (legh) by 3.01, 1.25, and 1.22 times, respectively, compared to the original strain.
- The innovation provides new signal peptides that enhance the expression level of leghemoglobin, offering a novel approach to improving gene expression in genetic engineering.
Potential Applications: The technology can be applied in biotechnology, agriculture, and pharmaceutical industries for enhancing gene expression levels in various organisms.
Problems Solved: The technology addresses the challenge of low gene expression levels in recombinant strains, providing a solution to improve protein production efficiency.
Benefits: - Increased expression levels of leghemoglobin - Enhanced efficiency in gene expression - Potential for improved protein production in various applications
Commercial Applications: "Enhanced Gene Expression Technology for Improved Protein Production in Biotechnology and Pharmaceutical Industries"
Questions about gene expression cassette encoding hemoglobin: 1. How does the use of signal peptides impact gene expression levels? 2. What are the potential implications of increased leghemoglobin expression in various industries?
Frequently Updated Research: Stay updated on advancements in genetic engineering techniques for enhancing gene expression levels and protein production efficiency.
Original Abstract Submitted
the present disclosure provides a gene expression cassette encoding hemoglobin (hb) and use thereof, and relates to the technical field of genetic engineering. in the present disclosure, a recombinant strain with signal peptides pel b, pho a, and omp a inserted under same conditions has an expression level of leghemoglobin (legh) increased by 3.01, 1.25, and 1.22 times, respectively, compared with an original legh expressing strain. the present disclosure provides signal peptides that can increase the expression level of the legh, and provide a new idea for research and application of improving the expression level of the legh.
