Yunnan Agricultural University (20240276958). SGRNA AND CONSTRUCTING DUAL PIG MODEL OF SEVERE IMMUNODEFICIENCY AND LIVER INJURY AND USE THEREOF simplified abstract
Contents
SGRNA AND CONSTRUCTING DUAL PIG MODEL OF SEVERE IMMUNODEFICIENCY AND LIVER INJURY AND USE THEREOF
Organization Name
Yunnan Agricultural University
Inventor(s)
Hong-jiang Wei of Kunming (CN)
Qing-feng Chen of Kunming (CN)
SGRNA AND CONSTRUCTING DUAL PIG MODEL OF SEVERE IMMUNODEFICIENCY AND LIVER INJURY AND USE THEREOF - A simplified explanation of the abstract
This abstract first appeared for US patent application 20240276958 titled 'SGRNA AND CONSTRUCTING DUAL PIG MODEL OF SEVERE IMMUNODEFICIENCY AND LIVER INJURY AND USE THEREOF
The present disclosure involves the creation of a dual pig model of severe immunodeficiency and liver injury using a sgRNA and CRISPR/Cas9 technology.
- Knocking out rag2, il2rβ, and fah genes in a porcine fetal fibroblast using CRISPR/Cas9 technology.
- Constructing a rag2/il2rβ/fah triple-gene edited cloned pig through somatic cell nuclear transfer technology.
- Obtaining a dual pig model of severe immunodeficiency and liver injury through phenotypic analysis and identification.
Potential Applications: - Tumor biology research - Cell transplantation studies - Humanized animal model development
Problems Solved: - Long production period - Low efficiency - Irreversible damage - Unsatisfactory humanization degree
Benefits: - Batch construction of dual pig models - Continuous cloning technology - Great market application prospects
Commercial Applications: Title: "Innovative Dual Pig Model for Immunodeficiency and Liver Injury Research" Description: This technology can be utilized in pharmaceutical research, biotechnology companies, and academic institutions for studying various diseases and treatments.
Questions about the technology: 1. How does this dual pig model compare to existing animal models for research purposes? 2. What are the potential ethical considerations surrounding the use of genetically modified animals in research?
Frequently Updated Research: Stay updated on advancements in CRISPR/Cas9 technology for gene editing in animal models to enhance the efficiency and accuracy of creating dual pig models for research purposes.
Original Abstract Submitted
the present disclosure relates to a sgrna and constructing a dual pig model of severe immunodeficiency and liver injury and use thereof. the method comprises the steps of knocking out rag2, il2r� and fah genes in a porcine fetal fibroblast by using a crispr/cas9 technology, constructing a rag2/il2r�/fah triple-gene edited cloned pig by using a somatic cell nuclear transfer technology, and obtaining a dual pig model of severe immunodeficiency and liver injury through phenotypic analysis and identification. the method overcomes the problems of long production period, low efficiency, irreversible damage, unsatisfactory use in a humanization degree and the like in the existing model construction technology, can realize a batch construction of the dual pig model of severe immunodeficiency and liver injury by a continuous cloning technology, and has great advantages and potential market application prospects in the related fields of tumor biology, cell transplantation, humanized animal models and the like.
