US Patent Application 18315370. MICROFLUDIC LAB-ON-CHIP DEVICE, MATRIX, SMALL MOLECULES AND THREE-DIMENSIONAL SPHEROIDS FOR CELL REPROGRAMMING simplified abstract
MICROFLUDIC LAB-ON-CHIP DEVICE, MATRIX, SMALL MOLECULES AND THREE-DIMENSIONAL SPHEROIDS FOR CELL REPROGRAMMING
Organization Name
The Regents of the University of California
Inventor(s)
Song Li of Beverly Hills CA (US)
Yang Song of Los Angeles CA (US)
Jennifer Soto of Santa Monica CA (US)
LeeAnn Li of Los Angeles CA (US)
Binru Chen of Los Angeles CA (US)
Yifan Wu of Los Angeles CA (US)
MICROFLUDIC LAB-ON-CHIP DEVICE, MATRIX, SMALL MOLECULES AND THREE-DIMENSIONAL SPHEROIDS FOR CELL REPROGRAMMING - A simplified explanation of the abstract
This abstract first appeared for US patent application 18315370 titled 'MICROFLUDIC LAB-ON-CHIP DEVICE, MATRIX, SMALL MOLECULES AND THREE-DIMENSIONAL SPHEROIDS FOR CELL REPROGRAMMING
Simplified Explanation
- The patent application is about an invention that improves cellular reprogramming and gene editing techniques in biology. - The invention overcomes the challenges of low and variable efficiencies in these technologies. - It provides optimized methods and systems for cellular engineering and gene editing. - The invention includes microfluidic devices, chemical treatment, cell adhesion manipulation, and 3D spheroid culture. - These techniques modulate epigenetic changes and significantly enhance cell reprogramming and gene editing. - The changes in genome-wide chromatin accessibility caused by the invention can guide gene silencing, activation, insertion, and editing.
Original Abstract Submitted
Cellular reprogramming and gene editing represent major advancements in biology, and has wide applications in regenerative medicine, disease therapy and drug screening. However, low and variable efficiencies have created significant roadblocks to the full application of these technologies. The invention disclosed herein overcomes these roadblocks by providing optimized methods and systems that are useful in a variety of cellular engineering and gene editing methodologies, including for example methods designed to enhance the reprogramming of somatic cells into neural cells or pluripotent cells. The invention provides innovative microfluidic devices, chemical treatment, cell adhesion manipulation, and 3D spheroid culture to modulate epigenetic changes and significantly enhance cell reprogramming and gene editing; the genome-wide chromatin accessibility changes caused by cell nuclear deformation, 3D culture, decreased cell adhesions, and the reduction of intracellular tension can provide guidance for guided gene silencing, activation, insertion and/or editing.
