Robert bosch gmbh (20240240172). Process for Purifying Nucleic Acids, in particular in a Microfluidic Apparatus simplified abstract

Process for Purifying Nucleic Acids, in particular in a Microfluidic Apparatus

Organization Name

robert bosch gmbh

Inventor(s)

Tanja Maucher of Stuttgart (DE)

Christian Grumaz of Waiblingen (DE)

Martina Budde of Karlsruhe (DE)

Process for Purifying Nucleic Acids, in particular in a Microfluidic Apparatus - A simplified explanation of the abstract

This abstract first appeared for US patent application 20240240172 titled 'Process for Purifying Nucleic Acids, in particular in a Microfluidic Apparatus

Simplified Explanation:

This patent application discloses a method for purifying nucleic acids, particularly in a microfluidic apparatus. The method involves releasing nucleic acids from a sample using chaotrope substances in a transport medium and binding the nucleic acids to a filter, with the transport medium mixed with a wash buffer to adjust binding conditions. A microfluidic kit for implementing the method is also described.

• Key Features and Innovation:

   - Purification of nucleic acids in a microfluidic system.
   - Use of chaotrope substances to release nucleic acids from samples.
   - Binding nucleic acids to a filter for purification.
   - Adjustment of binding conditions by mixing transport medium with a wash buffer.
   - Provision of a microfluidic kit for carrying out the purification process.

• Potential Applications:

   - Biomedical research laboratories.
   - Clinical diagnostics for genetic testing.
   - Forensic analysis for DNA profiling.
   - Environmental testing for microbial identification.
   - Pharmaceutical industry for drug development.

• Problems Solved:

   - Efficient purification of nucleic acids.
   - Simplified process for handling nucleic acid samples.
   - Compatibility with microfluidic systems.
   - Enhanced sensitivity and accuracy in nucleic acid analysis.
   - Reduction of contamination risks during purification.

• Benefits:

   - Faster and more reliable nucleic acid purification.
   - Increased throughput in sample processing.
   - Improved quality of nucleic acid samples.
   - Cost-effective method for purification.
   - Automation potential for high-throughput applications.

• Commercial Applications:

   - Title: "Microfluidic Nucleic Acid Purification Method for Biomedical and Diagnostic Applications"
   - Potential commercial uses in research institutions, diagnostic laboratories, and biotechnology companies.
   - Market implications include improved efficiency in nucleic acid analysis, leading to faster results and better data quality.

• Prior Art:

   - Readers can explore prior patents related to microfluidic nucleic acid purification methods, chaotrope substances in nucleic acid extraction, and filter-based nucleic acid purification techniques.

• Frequently Updated Research:

   - Stay informed about advancements in microfluidic technologies for nucleic acid purification.
   - Follow developments in chaotrope-based methods for releasing nucleic acids from samples.
   - Monitor innovations in filter materials and designs for nucleic acid binding and purification.

Questions about Nucleic Acid Purification in Microfluidic Systems: 1. How does the use of chaotrope substances improve nucleic acid purification efficiency in microfluidic systems? 2. What are the potential challenges in scaling up this method for high-throughput applications?

Original Abstract Submitted

a method for purifying nucleic acids, in particular in a microfluidic apparatus, is disclosed. the method includes releasing nucleic acids from a sample by way of chaotrope substances in a transport medium and binding the nucleic acids to a filter, the transport medium being mixed with a first portion of a wash buffer in order to adjust the binding conditions. a microfluidic kit for carrying out the method is further disclosed.